RT Journal Article
SR Electronic
T1 A Single-Objective Light-Sheet Microscope with 200 nm-Scale Resolution.
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.04.07.030569
DO 10.1101/2020.04.07.030569
A1 Etai Sapoznik
A1 Bo-Jui Chang
A1 Robert J. Ju
A1 Erik S. Welf
A1 David Broadbent
A1 Alexandre F. Carisey
A1 Samantha J. Stehbens
A1 Kyung-min Lee
A1 Arnaldo Marín
A1 Ariella B. Hanker
A1 Jens C. Schmidt
A1 Carlos L. Arteaga
A1 Bin Yang
A1 Rory Kruithoff
A1 Doug P. Shepherd
A1 Alfred Millett-Sikking
A1 Andrew G. York
A1 Kevin M. Dean
A1 Reto Paul Fiolka
YR 2020
UL http://biorxiv.org/content/early/2020/04/09/2020.04.07.030569.abstract
AB We present a single-objective light-sheet microscope, also known as an oblique-plane microscope, that uses a bespoke glass-tipped tertiary objective and improves the resolution, field of view, usability, and stability over previous variants. Owing to its high numerical aperture optics, this microscope achieves the highest lateral resolution in light-sheet fluorescence microscopy, and its axial resolution is similar to that of Lattice Light-Sheet Microscopy. Given this performance, we demonstrate high-resolution imaging of clathrin-mediated endocytosis, vimentin, the endoplasmic reticulum, membrane dynamics, and natural killer cell-mediated cell death. Furthermore, we image biological phenomena that would be otherwise challenging or impossible to perform in a traditional light-sheet microscope geometry, including cell migration through a confined space within a microfluidic device, photoactivation of PI3K, and diffusion of cytoplasmic rheological tracers at a volumetric rate of 14 Hz.