TY - JOUR T1 - Inferring high-dimensional pathways of trait acquisition in evolution and disease JF - bioRxiv DO - 10.1101/409656 SP - 409656 AU - Sam F. Greenbury AU - Mauricio Barahona AU - Iain G. Johnston Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/09/05/409656.abstract N2 - The explosion of data throughout the sciences provides unprecedented opportunities to learn about the dynamics of evolution and disease progression. Here, we describe a highly generalisable statistical platform to infer the dynamic pathways by which many, potentially interacting, discrete traits are acquired or lost over time in biological processes. The platform uses HyperTraPS (hypercubic transition path sampling) to learn progression pathways from cross-sectional, longitudinal, or phylogenetically-linked data with unprecedented efficiency, readily distinguishing multiple competing pathways, and identifying the most parsimonious mechanisms underlying given observations. Its Bayesian structure quantifies uncertainty in pathway structure and allows interpretable predictions of behaviours, such as which symptom a patient will acquire next. We exploit the model’s topology to provide visualisation tools for intuitive assessment of multiple, variable pathways. We apply the method to ovarian cancer progression and the evolution of multidrug resistance in tuberculosis, demonstrating its power to reveal previously undetected dynamic pathways. ER -