PT - JOURNAL ARTICLE AU - Kateryna Trofymchuk AU - Viktorija Glembockyte AU - Lennart Grabenhorst AU - Florian Steiner AU - Carolin Vietz AU - Cindy Close AU - Martina Pfeiffer AU - Lars Richter AU - Max L. Schütte AU - Florian Selbach AU - Renukka Yaadav AU - Jonas Zähringer AU - Qingshan Wei AU - Aydogan Ozcan AU - Birka Lalkens AU - Guillermo P. Acuna AU - Philip Tinnefeld TI - Addressable Nanoantennas with Cleared Hotspots for Single-Molecule Detection on a Portable Smartphone Microscope AID - 10.1101/2020.04.09.032037 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.04.09.032037 4099 - http://biorxiv.org/content/early/2020/04/09/2020.04.09.032037.short 4100 - http://biorxiv.org/content/early/2020/04/09/2020.04.09.032037.full AB - The advent of highly sensitive photodetectors1,2 and the development of photostabilization strategies3 made detecting the fluorescence of a single molecule a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g. in point-of-care diagnostic settings, where costly equipment would be prohibitive.4 Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in the NACHOS emit up to 461-fold brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia "on the road “.Competing Interest StatementPT and GPA are inventors on a patent of the described Bottom-up method for fluorescence enhancement in molecular assays, EP1260316.1, 2012, US20130252825 A1.