TY - JOUR T1 - Structural Basis for the Inhibition of the RNA-Dependent RNA Polymerase from SARS-CoV-2 by Remdesivir JF - bioRxiv DO - 10.1101/2020.04.08.032763 SP - 2020.04.08.032763 AU - Wanchao Yin AU - Chunyou Mao AU - Xiaodong Luan AU - Dan-Dan Shen AU - Qingya Shen AU - Haixia Su AU - Xiaoxi Wang AU - Fulai Zhou AU - Wenfeng Zhao AU - Minqi Gao AU - Shenghai Chang AU - Yuan-Chao Xie AU - Guanghui Tian AU - He-Wei Jiang AU - Sheng-Ce Tao AU - Jingshan Shen AU - Yi Jiang AU - Hualiang Jiang AU - Yechun Xu AU - Shuyang Zhang AU - Yan Zhang AU - H. Eric Xu Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/04/09/2020.04.08.032763.abstract N2 - The pandemic of Corona Virus Disease 2019 (COVID-19) caused by SARS-CoV-2 has become a global crisis. The replication of SARS-CoV-2 requires the viral RNA-dependent RNA polymerase (RdRp), a direct target of the antiviral drug, Remdesivir. Here we report the structure of the SARS-CoV-2 RdRp either in the apo form or in complex with a 50-base template-primer RNA and Remdesivir at a resolution range of 2.5-2.8 Å. The complex structure reveals that the partial double-stranded RNA template is inserted into the central channel of the RdRp where Remdesivir is incorporated into the first replicated base pair and terminates the chain elongation. Our structures provide critical insights into the working mechanism of viral RNA replication and a rational template for drug design to combat the viral infection.Competing Interest StatementThe authors have declared no competing interest. ER -