RT Journal Article
SR Electronic
T1 Improved synthetic lipidation-based protein translocation system for SNAP-tag fusion proteins
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.04.09.035188
DO 10.1101/2020.04.09.035188
A1 Tatsuyuki Yoshii
A1 Kai Tahara
A1 Sachio Suzuki
A1 Yuka Hatano
A1 Keiko Kuwata
A1 Shinya Tsukiji
YR 2020
UL http://biorxiv.org/content/early/2020/04/10/2020.04.09.035188.abstract
AB The ability to artificially attach lipids to specific intracellular protein targets would be a valuable approach for controlling protein localization and function in cells. We recently devised a chemogenetic method in which a SNAP-tag fusion protein can be translocated from the cytoplasm to the plasma membrane by post-translationally and covalently conjugating a synthetic lipopeptide in cells. However, the first-generation system lacked general applicability. Herein, we present an improved synthetic lipidation system that enables efficient plasma membrane translocation of SNAP-tag fusion proteins in cells. This second-generation system is now applicable to the control of various cell-signaling molecules, offering a new and useful research tool in chemical biology and synthetic biology.Competing Interest StatementT.Y., S.S., and S.T. are co-inventors on Japan patent application No. 2020-030868 that includes the mDc motif described in this paper. Other authors declare no competing interests.