RT Journal Article
SR Electronic
T1 Single-cell transcriptional dynamics and origins of neuronal diversity in the developing mouse neocortex
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 409458
DO 10.1101/409458
A1 L. Telley
A1 G. Agirman
A1 J. Prados
A1 S. Fièvre
A1 P. Oberst
A1 I. Vitali
A1 L. Nguyen
A1 A. Dayer
A1 D. Jabaudon
YR 2018
UL http://biorxiv.org/content/early/2018/09/06/409458.abstract
AB During cortical development, distinct subtypes of glutamatergic neurons are sequentially born and differentiate from dynamic populations of progenitors. The neurogenic competence of these progenitors progresses as corticogenesis proceeds; likewise, newborn neurons transit through sequential states as they differentiate. Here, we trace the developmental transcriptional trajectories of successive generations of apical progenitors (APs) and isochronic cohorts of their daughter neurons using parallel single-cell RNA sequencing between embryonic day (E) 12 and E15 in the mouse cerebral cortex. Our results identify the birthdate- and differentiation stage-related transcriptional dynamics at play during corticogenesis. As corticogenesis proceeds, APs transit through embryonic age-dependent molecular states, which are transmitted to their progeny to generate successive initial daughter cell identities. In neurons, essentially conserved post-mitotic differentiation programs are applied onto these distinct AP-derived ground states, allowing temporally-regulated sequential emergence of specialized neuronal cell types. Molecular temporal patterning of sequentially-born daughter neurons by their respective mother cell thus underlies emergence of neuronal diversity in the neocortex.One Sentence Summary During corticogenesis, temporally dynamic molecular birthmarks are transmitted from progenitors to their post-mitotic progeny to generate neuronal diversity.