RT Journal Article SR Electronic T1 Dynamic 3D tissue architecture directs BMP morphogen signaling during Drosophila wing morphogenesis JF bioRxiv FD Cold Spring Harbor Laboratory SP 411009 DO 10.1101/411009 A1 Jinghua Gui A1 Yunxian Huang A1 Martin Kracklauer A1 Daniel Toddie-Moore A1 Kenji Kikushima A1 Stephanie Nix A1 Yukitaka Ishimoto A1 Osamu Shimmi YR 2018 UL http://biorxiv.org/content/early/2018/09/06/411009.abstract AB At the level of organ formation, tissue morphogenesis drives developmental processes in animals, often involving the rearrangement of two-dimensional (2D) structures into more complex three-dimensional (3D) tissues. These processes can be directed by growth factor signaling pathways. However, little is known about how such morphological changes affect the spatiotemporal distribution of growth factor signaling. Here, using the Drosophila pupal wing, we address how Decapentaplegic (Dpp) / Bone Morphogenetic Protein (BMP) signaling and 3D wing morphogenesis are coupled. Dpp, expressed in the longitudinal veins (LVs) of the pupal wing, initially diffuses laterally during the inflation stage to regulate cell proliferation. Dpp localization is then refined to the LVs within each epithelial plane, but with active interplanar signaling for vein patterning, as the two epithelia appose. Our data further suggest that the 3D architecture of the wing epithelia directs the spatial distribution of BMP signaling, revealing how 3D morphogenesis is an emergent property of the interactions between extracellular signaling and tissue shape changes.