RT Journal Article
SR Electronic
T1 Evaluation of heating and chemical protocols for inactivating SARS-CoV-2
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.04.11.036855
DO 10.1101/2020.04.11.036855
A1 Boris Pastorino
A1 Franck Touret
A1 Magali Gilles
A1 Xavier de Lamballerie
A1 Remi N. Charrel
YR 2020
UL http://biorxiv.org/content/early/2020/04/11/2020.04.11.036855.abstract
AB Clinical samples collected in COVID-19 patients are commonly manipulated in BSL-2 laboratories for diagnostic purpose. We used the French norm NF-EN-14476+A2 derived from the European standard EN-14885. To avoid the risk of exposure of laboratory workers, we showed that Sodium-dodecyl-sulfate and Triton-X100 must be added to guanidinium thiocyanate-lysis buffers to obtain a 6-log reduction of infectious virus. Heating protocol consisting of 92°C-15min was more effective rather than 56°C-30min and 60°C-60min to achieve 6-log reduction.Competing Interest StatementThe authors have declared no competing interest.