RT Journal Article
SR Electronic
T1 Detection of <em>Wolbachia</em> in Field-Collected Mosquito Vector, <em>Aedes aegypti</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 408856
DO 10.1101/408856
A1 Carvajal, Thaddeus M.
A1 Hashimoto, Kazuki
A1 Harnandika, Reza Kurniawan
A1 Amalin, Divina M
A1 Watanabe, Kozo
YR 2018
UL http://biorxiv.org/content/early/2018/09/08/408856.abstract
AB It was the impression from past literature that Wolbachia is not naturally found in Ae. aegypti. However, there are have been reports that recently reveals the presence of this endosymbiont in this mosquito vector. With this, our study presents additional support of Wolbachia infection in Ae. aegypti by screening field-collected adult mosquitoes using Wolbachiaspecific 16S rDNA and its surface protein (wsp) makers under optimized PCR conditions. From a total of 672 Ae. aegpyti adult mosquito samples collected in Metropolitan Manila, Philippines, 113 (16.8%) and 89 (13.2%) individual mosquito samples were determined to be Wolbachia infected using the wsp and 16S rDNA markers, respectively. The Ae. aegpyti wsp sample sequences were similar or identical to five known Wolbachia strains belonging to supergroups A or B while majority of 16S rDNA sample sequences were similar to strains belonging to supergroup B. Overall, 80 (11.90%) individual mosquito samples revealed to show positive amplifications in both markers and 69.0% showed congruence in supergroup identification (supergroup B). Our findings illustrate that the infection status of Wolbachia in Ae. aegypti may appear common than previously recognized.