PT  - JOURNAL ARTICLE
AU  - Jackson Champer
AU  - Joan Chung
AU  - Yoo Lim Lee
AU  - Chen Liu
AU  - Emily Yang
AU  - Zhaoxin Wen
AU  - Andrew G. Clark
AU  - Philipp W. Messer
TI  - Molecular safeguarding of CRISPR gene drive experiments
AID  - 10.1101/411876
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 411876
4099  - http://biorxiv.org/content/early/2018/09/08/411876.short
4100  - http://biorxiv.org/content/early/2018/09/08/411876.full
AB  - CRISPR-based gene drives have sparked both enthusiasm and deep concerns due to their potential for genetically altering entire species. This raises the question about our ability to prevent the unintended spread of such drives from the laboratory into a natural population. Here, we experimentally demonstrate the suitability of synthetic target sites and split drives as flexible safeguarding strategies for gene drive experiments.