TY - JOUR T1 - Specific PIP<sub>2</sub> Binding Promotes Calcium Activation of TMEM16A Chloride Channels JF - bioRxiv DO - 10.1101/2020.04.13.039743 SP - 2020.04.13.039743 AU - Zhiguang Jia AU - Jianhan Chen Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/04/14/2020.04.13.039743.abstract N2 - TMEM16A is a widely expressed Ca2+-activated Cl- channel that regulates crucial physiological functions including fluid secretion, neuronal excitability, and smooth muscle contraction. There is a critical need to understand the molecular mechanisms of TMEM16A gating and regulation. However, high-resolution TMEM16A structures have failed to reveal an activated state with unobstructed permeation pathway even with saturating Ca2+. This has been attributed to the requirement of PIP2 for preventing TMEM16A desensitization. Here, we show that specific binding PIP2 to TMEM16A can lead to spontaneous opening of the permeation pathway in the Ca2+-bound state. The predicted activated state is highly consistent with a wide range of mutagenesis and functional data. It yields a maximal Cl- conductance of ~1 pS, similar to experimental estimates, and recapitulates the selectivity of larger SCN- over Cl-. The resulting molecular mechanism of activation provides a basis for understanding the interplay of multiple signals in controlling TMEM16A channel function.Competing Interest StatementThe authors have declared no competing interest. ER -