TY - JOUR T1 - Charged Gram-positive species sequester and decrease the potency of pediocin PA-1 in mixed microbial settings JF - bioRxiv DO - 10.1101/2020.04.13.039628 SP - 2020.04.13.039628 AU - Vikas D. Trivedi AU - Nikhil U. Nair Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/04/14/2020.04.13.039628.abstract N2 - Antimicrobial peptides (AMPs) have gained attention recently due to increasing antibiotic resistance amongst pathogens. Most AMPs are cationic in nature and their preliminary interactions with the negatively charged cell surface is mediated by electrostatic attraction. This is followed by pore formation, which is either receptor-dependent or -independent and leads to cell death. Typically, AMPs are characterized by their killing activity using bioactivity assays to determine host range and degree of killing. However, cell surface binding is independent from killing. Most of the studies performed to-date have attempted to quantify the peptide binding using artificial membranes. Here, we use the narrow-spectrum class IIa bacteriocin AMP pediocin PA-1 conjugated to a fluorescent dye as a probe to monitor cell surface binding. We developed a flow cytometry-based assay to quantify the strength of binding in target and non-target species. Through our binding assays, we found a strong positive correlation between cell surface charge and pediocin PA-1 binding. Interestingly, we also found inverse correlation between zeta potential and pediocin PA-1 binding, the correlation coefficient for which improved when only Gram-positives were considered. We also show the effect of the presence of protein, salt, polycationic species, and other non-target species on the binding of pediocin PA-1 to the target organism. We conclude that the of presence of highly charged non-target species, as well as solutes, can decrease the binding, and the apparent potency, of pediocin PA-1. Thus, these outcomes are highly significant to the use of pediocin PA-1 and related AMPs in mixed microbial settings such as those found in the gut microbiota.Competing Interest StatementThe authors have declared no competing interest. ER -