RT Journal Article
SR Electronic
T1 The CD56bright CD62L+ NKG2A+ immature cell subset is dominantly expanded in human cytokine-induced memory-like NK cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 405134
DO 10.1101/405134
A1 Juhyun Jin
A1 Yong-Oon Ahn
A1 Tae Min Kim
A1 Bhumsuk Keam
A1 Dong-Wan Kim
A1 Dae Seog Heo
YR 2018
UL http://biorxiv.org/content/early/2018/09/09/405134.abstract
AB Recent studies have revealed immunological memory of NK cells. Short-term in vitro cytokine stimulation also induces NK cell memory, but heterogeneous cell subsets within the cytokine-induced memory-like (CIML) NK cells has not been elucidated. Here we found that the dominant cell subset in human CIML NK cells are immature CD56bright CD62L+cells, and they were selectively expanded CD56bright CD16- CD62L+ NK cells. Although these cells acquired KIR expression after the cytokine stimulation, sustained NKG2A expression inhibits cytotoxicity against HLA-E+ target cells. In contrast, another checkpoint molecule LAG-3 is induced mainly on KIR+ NKG2C+ minor CIML NK cells. Our findings imply targeting NKG2A and LAG-3 should be considered for CIML NK cell-based immunotherapy.