RT Journal Article
SR Electronic
T1 Dietary supplementation with products of Citrus reticulata "Chachi" for improving the fecal microbiome of weaned piglets
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.04.14.040881
DO 10.1101/2020.04.14.040881
A1 Huafeng Lin
A1 Gang Li
A1 Haizhen Wang
A1 Lili Li
A1 Xun Chen
A1 Lei Shi
A1 Yong Tang
A1 Yanlei Chang
A1 Jie Yang
A1 Yuqi Liu
A1 Hanyue Gong
YR 2020
UL http://biorxiv.org/content/early/2020/04/14/2020.04.14.040881.abstract
AB Nutritional interventions play a critical role in modifying the intestinal microbiome of host animals. This study was conducted to interrogate the physiological effects on fecal microflora of weaned piglets via the dietary supplemented with two types of products of Citrus reticulata "Chachi", respectively. For this purpose, A total of 72 piglets with uniform sizes were randomly assigned to four dietary treatment groups consisted of a negative control group (NCG), a fermented citrus reticulata "Chachi" pulp group (FCRPG), a Citri Reticulatae Pericarpium group (CRPG) and a positive control group (PCG) in a 21-day feeding trial. After the raising experiment, fresh feces of piglets were analyzed systematically using multi-omics technologies. Metagenomics method with high-throughput compositional characterization indicated that the architecture and diversity of fecal microbiome were both influenced by these two additives and compound antibiotics. Metabolite analysis showed that FCRPG have an significant effects on fecal short-chain fatty acids (SCFAs) among four treatment groups. Results of functional proteomics approaches found that FCRPG presented the highest butyrate metabolic level, and CRPG showed the highest flavone and flavonol biosynthesis level in feces. In addition, NCP produced an effective effect on adjusting fecal microbiome proflie. Consequently, our findings demonstrate that dietary supplementation with FCRP or CRP modulates the microbial taxa, metabolic and proteomic alterations in fecal microbiota of weaned piglets for health maintaining.