PT  - JOURNAL ARTICLE
AU  - Jonathan Arias-Fuenzalida
AU  - Javier Jarazo
AU  - Jonas Walter
AU  - Gemma Gomez-Giro
AU  - Julia I. Forster
AU  - Paul M.A. Antony
AU  - Rejko Krueger
AU  - Jens C. Schwamborn
TI  - Automated high-throughput high-content autophagy and mitophagy analysis platform
AID  - 10.1101/412957
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 412957
4099  - http://biorxiv.org/content/early/2018/09/10/412957.short
4100  - http://biorxiv.org/content/early/2018/09/10/412957.full
AB  - Autophagy and mitophagy play a central role in cellular homeostasis. In pathological conditions, the flow of autophagy and mitophagy can be affected at multiple and distinct steps of the pathways. Unfortunately, the level of detail of current state of the art analyses does not allow detection or dissection of pathway intermediates. Moreover, is conducted in low-throughput manner on bulk cell populations. Defining autophagy and mitophagy pathway intermediates in a high-throughput manner is technologically challenging, and has not been addressed so far. Here, we overcome those limitations and developed a novel high-throughput phenotyping platform with automated high-content image analysis to assess autophagy and mitophagy pathway intermediates.