RT Journal Article
SR Electronic
T1 Automated high-throughput high-content autophagy and mitophagy analysis platform
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 412957
DO 10.1101/412957
A1 Jonathan Arias-Fuenzalida
A1 Javier Jarazo
A1 Jonas Walter
A1 Gemma Gomez-Giro
A1 Julia I. Forster
A1 Paul M.A. Antony
A1 Rejko Krueger
A1 Jens C. Schwamborn
YR 2018
UL http://biorxiv.org/content/early/2018/09/10/412957.abstract
AB Autophagy and mitophagy play a central role in cellular homeostasis. In pathological conditions, the flow of autophagy and mitophagy can be affected at multiple and distinct steps of the pathways. Unfortunately, the level of detail of current state of the art analyses does not allow detection or dissection of pathway intermediates. Moreover, is conducted in low-throughput manner on bulk cell populations. Defining autophagy and mitophagy pathway intermediates in a high-throughput manner is technologically challenging, and has not been addressed so far. Here, we overcome those limitations and developed a novel high-throughput phenotyping platform with automated high-content image analysis to assess autophagy and mitophagy pathway intermediates.