TY - JOUR T1 - Butyrate producing Clostridiales utilize distinct human milk oligosaccharides correlating to early colonization and prevalence in the human gut JF - bioRxiv DO - 10.1101/2020.04.15.038927 SP - 2020.04.15.038927 AU - Michael Jakob Pichler AU - Chihaya Yamada AU - Bashar Shuoker AU - Maria Camila Alvarez-Silva AU - Aina Gotoh AU - Maria Louise Leth AU - Erwin Schoof AU - Toshihiko Katoh AU - Mikiyasu Sakanaka AU - Takane Katayama AU - Chunsheng Jin AU - Niclas G. Karlsson AU - Manimozhiyan Arumugam AU - Shinya Fushinobu AU - Maher Abou Hachem Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/04/15/2020.04.15.038927.abstract N2 - The early life human gut microbiota exerts life-long health effects on the host, but the mechanisms underpinning its assembly remain elusive. Particularly, the early colonization of Clostridiales from the Roseburia-Eubacterium group, associated with protection from colorectal cancer, immune- and metabolic disorders is enigmatic. Here we unveil the growth of Roseburia and Eubacterium members on human milk oligosaccharides (HMOs) using an unprecedented catabolic apparatus. The described HMO pathways and additional glycan utilization loci confer co-growth with Akkermansia muciniphilia via cross-feeding and access to mucin O-glycans. Strikingly, both, HMO and xylooligosaccharide pathways, were active simultaneously attesting an adaptation to a mixed HMO-solid food diet. Analyses of 4599 Roseburia genomes underscored the preponderance of HMO pathways and highlighted different HMO utilization phylotypes. Our revelations provide a possible rationale for the early establishment and resilience of butyrate producing Clostridiales and expand the role of specific HMOs in the assembly of the early life microbiota.Competing Interest StatementThe authors have declared no competing interest. ER -