RT Journal Article
SR Electronic
T1 “Degradation of the extracellular matrix is part of the pathology of ulcerative colitis”
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 412684
DO 10.1101/412684
A1 Kirov, Stefan
A1 Sasson, Ariella
A1 Zhang, Clarence
A1 Chasalow, Scott
A1 Dongre, Ashok
A1 Steen, Hanno
A1 Stensballe, Allan
A1 Andersen, Vibeke
A1 Birkelund, Svend
A1 Bennike, Tue Bjerg
YR 2018
UL http://biorxiv.org/content/early/2018/09/10/412684.abstract
AB The scientific value of re-analyzing existing datasets is often proportional to the complexity of the data. Proteomics data are inherently complex and can be analyzed at many levels, including proteins, peptides, and post-translational modifications to verify and/or develop new hypotheses. In this paper, we present our re-analysis of a previously published study comparing colon biopsy samples from ulcerative colitis (UC) patients to non-affected controls. In addition to confirming and reinforcing the original finding of upregulation of neutrophil extracellular traps (NETs), we report novel findings, including that Extracellular Matrix (ECM) degradation and neutrophil maturation are involved in the pathology of UC. The pharmaceutically most relevant differential protein expressions were confirmed using immunohistochemistry as an orthogonal method. As part of this study, we also compared proteomics data to previously published mRNA expression data. These comparisons indicated compensatory regulation at transcription levels of the ECM proteins we identified and open possible new venues for drug discovery.