RT Journal Article SR Electronic T1 RootPainter: Deep Learning Segmentation of Biological Images with Corrective Annotation JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.04.16.044461 DO 10.1101/2020.04.16.044461 A1 Abraham George Smith A1 Eusun Han A1 Jens Petersen A1 Niels Alvin Faircloth Olsen A1 Christian Giese A1 Miriam Athmann A1 Dorte Bodin Dresbøll A1 Kristian Thorup-Kristensen YR 2020 UL http://biorxiv.org/content/early/2020/04/18/2020.04.16.044461.abstract AB We present RootPainter, a GUI-based software tool for the rapid training of deep neural networks for use in biological image analysis. RootPainter facilitates both fully-automatic and semiautomatic image segmentation. We investigate the effectiveness of RootPainter using three plant image datasets, evaluating its potential for root length extraction from chicory roots in soil, biopore counting and root nodule counting from scanned roots. We also use RootPainter to compare dense annotations to corrective ones which are added during the training based on the weaknesses of the current model.Competing Interest StatementThe authors have declared no competing interest.