PT  - JOURNAL ARTICLE
AU  - Weiye Song
AU  - Wenjun Shao
AU  - Wei Yi
AU  - Rongrong Liu
AU  - Manishi Desai
AU  - Steven Ness
AU  - Ji Yi
TI  - Visible light optical coherence tomography angiography (vis-OCTA) and local microvascular retinal oximetry in human retina
AID  - 10.1101/2020.04.17.047514
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.04.17.047514
4099  - http://biorxiv.org/content/early/2020/04/18/2020.04.17.047514.short
4100  - http://biorxiv.org/content/early/2020/04/18/2020.04.17.047514.full
AB  - We report herein the first visible light optical coherence tomography angiography (vis-OCTA) for human retinal imaging. Compared to the existing vis-OCT systems, we devised a spectrometer with a narrower bandwidth to increase the spectral power density for OCTA imaging, while retaining the major spectral contrast in the blood. We achieved a 100 kHz A-line rate, the fastest acquisition speed reported so far for human retinal vis-OCT. We rigorously optimized the imaging protocol such that a single acquisition takes <6 seconds with a field of view (FOV) of 3×7.8 mm2. The angiography enables accurate localization of microvasculature down to the capillary level and thus enables oximetry at vessels < 100 μm in diameter. We demonstrated microvascular hemoglobin oxygen saturation (sO2) at the feeding and draining vessels at the perifoveal region. The longitudinal repeatability was assessed by <5% coefficient of variation (CV). The unique capabilities of our vis-OCTA system may allow studies on the role of microvascular oxygen in various retinal pathologies.Competing Interest StatementThe authors have declared no competing interest.