RT Journal Article
SR Electronic
T1 Comparison of SARS-CoV2 N gene real-time RT-PCR targets and commercially available mastermixes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.04.17.047118
DO 10.1101/2020.04.17.047118
A1 Julianne R Brown
A1 Denise O’Sullivan
A1 Rui PA Pereira
A1 Alexandra S Whale
A1 Eloise Busby
A1 Jim Huggett
A1 Kathryn Harris
YR 2020
UL http://biorxiv.org/content/early/2020/04/19/2020.04.17.047118.abstract
AB We aim to test four one-step RT real-time mastermix options for use in SARS-CoV2 real-time PCR, with three primer/probe assays targeting the N gene. The lower limit of detection is determined using a SARS CoV2 N gene RNA transcript dilution series (to 1 copy/µl) and verified using 74 nose and throat swabs.The N2 assay demonstrates the most sensitive detection of SARS-Cov-2 RNA. Three of the four mastermixes performed well, with the Takara One Step PrimeScript™ III RT-PCR Kit mastermix demonstrating improved performance at the lower limit of detection.Competing Interest StatementThe authors have declared no competing interest.