RT Journal Article
SR Electronic
T1 Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.04.18.047878
DO 10.1101/2020.04.18.047878
A1 Ihling, Christian
A1 Tänzler, Dirk
A1 Hagemann, Sven
A1 Kehlen, Astrid
A1 Hüttelmaier, Stefan
A1 Sinz, Andrea
YR 2020
UL http://biorxiv.org/content/early/2020/04/19/2020.04.18.047878.abstract
AB Mass spectrometry (MS) can deliver valuable diagnostic data that complements genomic information and allows us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. Our protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients.Image credit (left): Gerd Altmann, Pixabay License, https://pixabay.com/illustrations/corona-coronavirus-virus-covid-19-4959447Competing Interest StatementThe authors have declared no competing interest.