TY - JOUR T1 - Rapid development of an inactivated vaccine for SARS-CoV-2 JF - bioRxiv DO - 10.1101/2020.04.17.046375 SP - 2020.04.17.046375 AU - Qiang Gao AU - Linlin Bao AU - Haiyan Mao AU - Lin Wang AU - Kangwei Xu AU - Minnan Yang AU - Yajing Li AU - Ling Zhu AU - Nan Wang AU - Zhe Lv AU - Hong Gao AU - Xiaoqin Ge AU - Biao Kan AU - Yaling Hu AU - Jiangning Liu AU - Fang Cai AU - Deyu Jiang AU - Yanhui Yin AU - Chengfeng Qin AU - Jing Li AU - Xuejie Gong AU - Xiuyu Lou AU - Wen Shi AU - Dongdong Wu AU - Hengming Zhang AU - Lang Zhu AU - Wei Deng AU - Yurong Li AU - Jinxing Lu AU - Changgui Li AU - Xiangxi Wang AU - Weidong Yin AU - Yanjun Zhang AU - Chuan Qin Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/04/19/2020.04.17.046375.abstract N2 - The COVID-19 pandemic caused by SARS-CoV-2 has brought about an unprecedented crisis, taking a heavy toll on human health, lives as well as the global economy. There are no SARS-CoV-2-specific treatments or vaccines available due to the novelty of this virus. Hence, rapid development of effective vaccines against SARS-CoV-2 is urgently needed. Here we developed a pilot-scale production of a purified inactivated SARS-CoV-2 virus vaccine candidate (PiCoVacc), which induced SARS-CoV-2-specific neutralizing antibodies in mice, rats and non-human primates. These antibodies potently neutralized 10 representative SARS-CoV-2 strains, indicative of a possible broader neutralizing ability against SARS-CoV-2 strains circulating worldwide. Immunization with two different doses (3μg or 6 μg per dose) provided partial or complete protection in macaques against SARS-CoV-2 challenge, respectively, without any antibody-dependent enhancement of infection. Systematic evaluation of PiCoVacc via monitoring clinical signs, hematological and biochemical index, and histophathological analysis in macaques suggests that it is safe. These data support the rapid clinical development of SARS-CoV-2 vaccines for humans.One Sentence Summary A purified inactivated SARS-CoV-2 virus vaccine candidate (PiCoVacc) confers complete protection in non-human primates against SARS-CoV-2 strains circulating worldwide by eliciting potent humoral responses devoid of immunopathologyCompeting Interest StatementThe authors have declared no competing interest. ER -