PT - JOURNAL ARTICLE AU - Silva, Genivaldo Gueiros Z. AU - Dutilh, Bas E. AU - Edwards, Robert A. TI - FOCUS2: agile and sensitive classification of metagenomics data using a reduced database AID - 10.1101/046425 DP - 2016 Jan 01 TA - bioRxiv PG - 046425 4099 - http://biorxiv.org/content/early/2016/03/31/046425.short 4100 - http://biorxiv.org/content/early/2016/03/31/046425.full AB - Summary Metagenomics approaches rely on identifying the presence of organisms in the microbial community from a set of unknown DNA sequences. Sequence classification has valuable applications in multiple important areas of medical and environmental research. Here we introduce FOCUS2, an update of the previously published computational method FOCUS. FOCUS2 was tested with 10 simulated and 543 real metagenomes demonstrating that the program is more sensitive, faster, and more computationally efficient than existing methods.Availability The Python implementation is freely available at https://edwards.sdsu.edu/FOCUS2.Supplementary information available at Bioinformatics online.