RT Journal Article
SR Electronic
T1 CRISPRpic: Fast and precise analysis for <span class="underline">CRISPR</span>-induced mutations via <span class="underline">p</span>refixed <span class="underline">i</span>ndex <span class="underline">c</span>ounting
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 416768
DO 10.1101/416768
A1 Seung Woo Cho
A1 HoJoon Lee
A1 Howard Y. Chang
A1 Hanlee P. Ji
YR 2018
UL http://biorxiv.org/content/early/2018/09/13/416768.abstract
AB Analysis of CRISPR-induced mutations at targeted loci can be achieved by PCR amplification followed by massively parallel sequencing. We developed a novel algorithm, called CRISPRpic, to analyze sequencing reads from CRISPR experiments via counting exact-matches and pattern-searching. Compared to other methods that are based on sequence alignment, CRISPRpic provides precise mutation calling and ultrafast analysis of sequencing results. The Python script for CRISPRpic is available at https://github.com/compbio/CRISPRpic.List of abbreviationsNGSNext Generation SequencingCRISPRClustered Regularly Interspaced Short Palindromic RepeatsDSBdouble strand breaksgRNAsingle guide RNASpCas9Streptococcus pyogenes Cas9AsCpf1Acidaminococcus sp. Cpf1