TY - JOUR T1 - Time-resolved cryoEM using Spotiton JF - bioRxiv DO - 10.1101/2020.04.20.050518 SP - 2020.04.20.050518 AU - Venkata P. Dandey AU - William C. Budell AU - Hui Wei AU - Daija Bobe AU - Kashyap Maruthi AU - Mykhailo Kopylov AU - Edward Eng AU - Peter A. Kahn AU - Jenny E. Hinshaw AU - Nidhi Kundu AU - Crina M. Nimigean AU - Chen Fan AU - Nattakan Sukomon AU - Seth Darst AU - Ruth Saecker AU - James Chen AU - Brandon Malone AU - Clinton S. Potter AU - Bridget Carragher Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/04/21/2020.04.20.050518.abstract N2 - We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surface, and the mixing reaction stops when the grid is vitrified in liquid ethane, on the order of ~100 ms later. We demonstrate the utility of this approach for four biological systems where short-lived states are of high interest.Competing Interest StatementB.C./C.S.P. have a commercial relationship with STPL, a company that produces a commercially available instrument, Chameleon, that is based on the Spotiton prototype. ER -