TY - JOUR T1 - Agonist-induced Functional Analysis and Cell Sorting, a novel tool to select and analyze neurons: Fragile X as a proof of concept JF - bioRxiv DO - 10.1101/2020.04.20.050997 SP - 2020.04.20.050997 AU - Sara Castagnola AU - Julie Cazareth AU - Kevin Lebrigand AU - Marielle Jarjat AU - Virginie Magnone AU - Sebastien Delhaye AU - Frederic Brau AU - Barbara Bardoni AU - Thomas Maurin Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/04/21/2020.04.20.050997.abstract N2 - To get a better insight into the dynamic interaction between cells and their environment, we developed the agonist-induced Functional Analysis and Cell Sorting (aiFACS) technique, which allows the simultaneous recording and sorting of cells in real-time according to their immediate and individual response to a stimulus. By modulating the aiFACS selection parameters, testing different developmental times, using various stimuli and multiplying the analysis of readouts, it is possible to analyze cell populations of any tissue, including tumors. The association of aiFACS to single-cell transcriptomic allows to build a tissue cartography based on specific functional response/s of cells.As proof of concept, we used aiFACS on the dissociated mouse brain, a highly heterogenous tissue, enriching it in interneurons upon stimulation with an agonist of the glutamate receptors and upon sorting based on calcium levels. Further single-cell RNA-seq of these aiFACS-selected interneurons resulted in a nine-cluster classification. Furthermore, we used aiFACS on interneurons derived from the brain of the Fmr1-KO mouse, a rodent model of Fragile X syndrome. We show here that these interneurons manifest a generalized defective pharmacological response compared to wild type, affecting all the analyzed cell clusters at one specific post-natal developmental time.Competing Interest StatementThe authors have declared no competing interest. ER -