RT Journal Article
SR Electronic
T1 Comparative analysis of beneficial effects of Vancomycin treatment on Th1- and Th2-biased mice and role of gut microbiota
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 516898
DO 10.1101/516898
A1 Pratikshya Ray
A1 Uday Pandey
A1 Palok Aich
YR 2020
UL http://biorxiv.org/content/early/2020/04/22/516898.abstract
AB Aims Vancomycin, an antibiotic, is used to treat infection of multi-drug resistant strains of Clostridium difficile and Staphylococcus. Post-usage effects of vancomycin may lead to many unwanted effects including perturbation of gut microbiota. Perturbation of the gut microbiota, by Vancomycin, was used to understand the altered metabolic and innate immune profile of C57BL/6(Th1-biased) and BALB/c (Th2-biased) mice.Methods and Results Following treatment with vancomycin till day 4, we observed reduction in abundance of phyla Firmicutes and Bacteroides and increase in Proteobacteria in the gut for both strains of mice. Results further revealed a significant increase in the phylum Verrucomicrobia, from day 5 onwards following treatment with vancomycin led to decreased inflammation and increased rate of glucose tolerance in the host.Conclusions Continued dosage of vancomycin was more beneficial in C57BL/6 than BALB/c miceSignificance and Impact of the study The current study established that initial doses of vancomycin increased pathogenic bacteria but the continued doses of vancomycin provided significant health-related benefits to the host by decreasing pathogenic load and by increasing beneficial microbes of Verrucomicrobia phylum (A. muciniphila) more in C57BL/6 (Th-1) than BALB/c (Th-2) mice.Competing Interest StatementThe authors have declared no competing interest.