PT - JOURNAL ARTICLE AU - Amanda C. Smith AU - Meleah A. Hickman TI - Host association induces genome changes in <em>Candida albicans</em> which alters its virulence AID - 10.1101/2020.04.22.056002 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.04.22.056002 4099 - http://biorxiv.org/content/early/2020/04/23/2020.04.22.056002.short 4100 - http://biorxiv.org/content/early/2020/04/23/2020.04.22.056002.full AB - Candida albicans is an opportunistic fungal pathogen of humans that is typically diploid yet, has a highly labile genome that is tolerant of large-scale perturbations including chromosomal aneuploidy and loss-of-heterozygosity events. The ability to rapidly generate genetic variation is crucial for C. albicans to adapt to changing or stress environments, like those encountered in the host. Genetic variation occurs via stress-induced mutagenesis or can be generated through its parasexual cycle, which includes mating between diploids or stress-induced mitotic defects to produce tetraploids and non-meiotic ploidy reduction. However, it remains largely unknown how genetic background contributes to C. albicans genome instability in vitro or in vivo. Here, we tested how genetic background, ploidy and host environment impact C. albicans genome stability. We found that host association induced both loss-of-heterozygosity events and genome size changes, regardless of genetic background or ploidy. However, the magnitude and types of genome changes varied across C. albicans strains. We also assessed whether host-induced genomic changes resulted in any consequences on growth rate and virulence phenotypes and found that many host derived isolates had significant changes compared to their parental strains. Interestingly, host derivatives from diploid C. albicans predominantly displayed increased virulence, whereas host derivatives from tetraploid C. albicans had mostly reduced virulence. Together, these results are important for understanding how host-induced genomic changes in C. albicans alter the relationship between the host and C. albicans.Competing Interest StatementThe authors have declared no competing interest.