PT  - JOURNAL ARTICLE
AU  - Michael W. Dorrity
AU  - Christine Queitsch
AU  - Stanley Fields
TI  - High-throughput identification of dominant negative polypeptides in yeast
AID  - 10.1101/418608
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 418608
4099  - http://biorxiv.org/content/early/2018/09/15/418608.short
4100  - http://biorxiv.org/content/early/2018/09/15/418608.full
AB  - Dominant negative polypeptides can act as inhibitors by binding to the wild type protein or by titrating an essential ligand. Here, we use high-throughput sequencing of DNA libraries composed of fragments of yeast genes to identify dominant negative polypeptides based on their depletion during cell growth. The method uncovers numerous inhibitory polypeptides for a protein and thus is capable of defining interacting domains with exquisite resolution, even pinpointing individual residues that contact ligands.