RT Journal Article
SR Electronic
T1 Precise re-deposition of nucleosomes on repressive chromatin domains sustain epigenetic inheritance during DNA replication
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 418707
DO 10.1101/418707
A1 Thelma M. Escobar
A1 Ozgur Oksuz
A1 Nicolas Descostes
A1 Roberto Bonasio
A1 Danny Reinberg
YR 2018
UL http://biorxiv.org/content/early/2018/09/15/418707.abstract
AB Whether chromatin domains display localized strategies to transfer pre-existing nucleosomal (H3-H4)2 core histones and their post-translational modifications (PTMs) during DNA replication remains unknown, largely due to the limitations of direct and precise methods to follow the fate of parental nucleosomes behind the replication fork. Here, we devised an inducible, proximity-dependent labeling system to irreversibly mark replication-dependent H3.1 and H3.2 histones at desired loci in mouse embryonic stem cells such that their position before and after replication could be determined at high resolution. We found both local and non-local re-deposition of parental histones during DNA replication, with a ‘repressed’ chromatin state being locally preserved and an ‘active’ chromatin domain lacking such inheritance.One Sentence Summary A method that permanently labels histones at chosen loci revealed that nucleosomes from repressed (but not active) chromatin domains are re-deposited locally after DNA replication.