PT  - JOURNAL ARTICLE
AU  - Gabriela Huelgas-Morales
AU  - Mark Sanders
AU  - Gemechu Mekonnen
AU  - Tatsuya Tsukamoto
AU  - David Greenstein
TI  - Decreased torsinA or LINC Complex Function Rescues a Laminopathy in <em>Caenorhabditis elegans</em>
AID  - 10.1101/2020.04.22.055988
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.04.22.055988
4099  - http://biorxiv.org/content/early/2020/04/23/2020.04.22.055988.short
4100  - http://biorxiv.org/content/early/2020/04/23/2020.04.22.055988.full
AB  - The function of the nucleus depends on the integrity of the nuclear lamina, an intermediate filament network associated with the linker of nucleoskeleton and cytoskeleton (LINC) complex spanning the nuclear envelope. In turn, the AAA+ ATPase torsinA regulates force transmission from the cytoskeleton to the nucleus. In humans, mutations affecting nuclear envelope-associated proteins cause laminopathies, including progeria, myopathy, and dystonia. We report that decreasing the function of the C. elegans torsinA homolog, OOC-5, rescues the sterility and premature aging caused by a null mutation in the single worm lamin homolog, lmn-1. Loss of OOC-5 activity prevents nuclear collapse in lmn-1 mutants by disrupting the function of the LINC complex. These results suggest that LINC complex-transmitted forces damage nuclei with a compromised nuclear lamina.One Sentence Summary Inhibiting LINC complex activity prevents a progeric syndrome in C. elegans.Competing Interest StatementThe authors have declared no competing interest.