%0 Journal Article %A Konstantin Barylyuk %A Ludek Koreny %A Huiling Ke %A Simon Butterworth %A Oliver M. Crook %A Imen Lassadi %A Vipul Gupta %A Eelco Tromer %A Tobias Mourier %A Tim J. Stevens %A Lisa M. Breckels %A Arnab Pain %A Kathryn S. Lilley %A Ross F. Waller %T A subcellular atlas of Toxoplasma reveals the functional context of the proteome %D 2020 %R 10.1101/2020.04.23.057125 %J bioRxiv %P 2020.04.23.057125 %X Apicomplexan parasites cause major human disease and food insecurity. They owe their considerable success to novel, highly specialized cell compartments and structures. These adaptations drive their recognition and non-destructive penetration of host’s cells and the elaborate reengineering of these cells to promote growth, dissemination, and the countering of host defenses. The evolution of unique apicomplexan cellular compartments is concomitant with vast proteomic novelty that defines these new cell organizations and their functions. Consequently, half of apicomplexan proteins are unique and uncharacterized, and these cells are, therefore, very poorly understood. Here, we determine the steady-state subcellular location of thousands of proteins simultaneously within the globally prevalent apicomplexan parasite Toxoplasma gondii. This provides unprecedented comprehensive molecular definition to these cells and their novel compartments, and these data reveal the spatial organizations of protein expression and function, adaptation to hosts, and the underlying evolutionary trajectories of these pathogens.Competing Interest StatementThe authors have declared no competing interest. %U https://www.biorxiv.org/content/biorxiv/early/2020/04/23/2020.04.23.057125.full.pdf