TY - JOUR T1 - The Dlg-module and clathrin-mediated endocytosis regulate EGFR signaling and cyst cell-germline coordination in the <em>Drosophila</em> testis JF - bioRxiv DO - 10.1101/419937 SP - 419937 AU - Fani Papagiannouli AU - Cameron Wynn Berry AU - Margaret T. Fuller Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/09/17/419937.abstract N2 - Tissue homeostasis and repair relies on proper communication of stem cells and their differentiating daughters with the local tissue microenvironment. In the Drosophila male germline adult stem cell lineage, germ cells proliferate and progressively differentiate enclosed in supportive somatic cyst cells, forming a small organoid, the functional unit of differentiation. Here we show that cell polarity and vesicle trafficking influence signal transduction in cyst cells, with profound effects on the germ cells they enclose. Our data suggest that both the cortical components Dlg, Scrib, Lgl and the clathrin-mediated endocytic (CME) machinery downregulate EGFR signaling. Knockdown of dlg, scrib, lgl or CME components in cyst cells resulted in germ cell death, similar to increased signal transduction via the EGFR, while lowering EGFR or downstream signaling components rescued the defects. This work provides new insights on how cell polarity and endocytosis cooperate to regulate signal transduction and sculpt developing tissues.AbbreviationsCySCssomatic cyst stem cellsCCscyst cellsGSCsgermline stem cellsdlgdiscs largescribscribblelgllethal (2) giant larvaeCMEclathrin-mediated endocytosisChcclathrin heavy chain; shi, shibirePIP2PtdIns(4,5)P2. ER -