PT  - JOURNAL ARTICLE
AU  - Clowsley, Alexander H.
AU  - Kaufhold, William T.
AU  - Lutz, Tobias
AU  - Meletiou, Anna
AU  - Di Michele, Lorenzo
AU  - Soeller, Christian
TI  - Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy
AID  - 10.1101/2020.04.24.059410
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.04.24.059410
4099  - http://biorxiv.org/content/early/2020/04/25/2020.04.24.059410.short
4100  - http://biorxiv.org/content/early/2020/04/25/2020.04.24.059410.full
AB  - DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA ‘imagers’ to target epitopes. Its performance in biological samples is often constrained by spurious signals produced by excess imagers. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution.Competing Interest StatementThe authors have declared no competing interest.