RT Journal Article
SR Electronic
T1 Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.04.24.059410
DO 10.1101/2020.04.24.059410
A1 Clowsley, Alexander H.
A1 Kaufhold, William T.
A1 Lutz, Tobias
A1 Meletiou, Anna
A1 Di Michele, Lorenzo
A1 Soeller, Christian
YR 2020
UL http://biorxiv.org/content/early/2020/04/25/2020.04.24.059410.abstract
AB DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA ‘imagers’ to target epitopes. Its performance in biological samples is often constrained by spurious signals produced by excess imagers. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution.Competing Interest StatementThe authors have declared no competing interest.