PT  - JOURNAL ARTICLE
AU  - McKay, Paul F.
AU  - Hu, Kai
AU  - Blakney, Anna K.
AU  - Samnuan, Karnyart
AU  - Bouton, Clément R.
AU  - Rogers, Paul
AU  - Polra, Krunal
AU  - Lin, Paulo J.C.
AU  - Barbosa, Christopher
AU  - Tam, Ying
AU  - Shattock, Robin J.
TI  - Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine induces equivalent preclinical antibody titers and viral neutralization to recovered COVID-19 patients
AID  - 10.1101/2020.04.22.055608
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.04.22.055608
4099  - http://biorxiv.org/content/early/2020/04/25/2020.04.22.055608.short
4100  - http://biorxiv.org/content/early/2020/04/25/2020.04.22.055608.full
AB  - The spread of the SARS-CoV-2 into a global pandemic within a few months of onset motivates the development of a rapidly scalable vaccine. Here, we present a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle as a vaccine and demonstrate induction of robust neutralization of a pseudo-virus, proportional to quantity of specific IgG and of higher quantities than recovered COVID-19 patients. These data provide insight into the vaccine design and evaluation of immunogenicity to enable rapid translation to the clinic.Competing Interest StatementThe authors have declared no competing interest.