PT  - JOURNAL ARTICLE
AU  - Barbora Lavickova
AU  - Sebastian J. Maerkl
TI  - A simple, robust, and low-cost method to produce the PURE cell - free system
AID  - 10.1101/420570
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 420570
4099  - http://biorxiv.org/content/early/2018/09/18/420570.short
4100  - http://biorxiv.org/content/early/2018/09/18/420570.full
AB  - We demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription-translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 µg/mL at a cost of 0.09 USD/µL, leading to a 14-fold improvement in cost normalized protein synthesis yield over existing PURE systems. The OnePot method makes the PURE system easy to generate and allows it to be readily optimized and modified.