%0 Journal Article %A Christopher R. Arnette %A Jennifer L. Koetsier %A Joshua A. Broussard %A Pedram Gerami %A Jodi L. Johnson %A Kathleen J. Green %T Keratinocyte desmoglein 1 regulates the epidermal microenvironment and tanning response %D 2018 %R 10.1101/423269 %J bioRxiv %P 423269 %X Coordinated responses to environmental stimuli within the keratinocyte:melanocyte niche are poorly understood. Desmoglein 1 (Dsg1), a keratinocyte-specific desmosomal cell-cell adhesion protein with emerging signaling roles, is reduced by ultraviolet light radiation. Loss-of-function Dsg1 mutations elevate keratinocyte cytokines in Severe dermatitis, multiple Allergies, and Metabolic wasting (SAM) syndrome. We asked whether Dsg1 regulates keratinocyte:melanocyte paracrine communication to induce the tanning response. Dsg1-silenced keratinocytes increased Pro-opiomelanocortin mRNA and cytokine secretion. Melanocytes treated with conditioned media from Dsg1-silenced keratinocytes exhibited increased Mitf and Trp1 mRNA, melanin secretion, and dendrite length. Inhibiting the melanocyte pigment-associated melanocortin 1 receptor reduced pigment secretion in response to Dsg1-deficient conditioned media. Melanocytes incorporated into Dsg1-deficient human skin equivalents relocalized suprabasally, reminiscent of early melanoma pagetoid behavior. Dsg1 decreased in keratinocytes surrounding dysplastic nevi and early melanoma, but not benign nevi. We posit Dsg1 controls keratinocyte:melanocyte communication through paracrine signaling, which goes awry upon Dsg1 loss in melanoma development.AbbreviationsKCKeratinocyteMCMelanocyteDsg1Desmoglein 1UVUltraviolet lightE-cadEpithelial cadherinSAMSevere dermatitis, multiple allergies, and metabolic wasting %U https://www.biorxiv.org/content/biorxiv/early/2018/09/20/423269.full.pdf