RT Journal Article
SR Electronic
T1 Developmental Divergence of Sensory Stimulus Representation in Cortical Interneurons
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.04.28.065680
DO 10.1101/2020.04.28.065680
A1 Rahel Kastli
A1 Rasmus Vighagen
A1 Alexander van der Bourg
A1 Ali Ozgur Argunsah
A1 Asim Iqbal
A1 Fabian F. Voigt
A1 Daniel Kirschenbaum
A1 Adriano Aguzzi
A1 Fritjof Helmchen
A1 Theofanis Karayannis
YR 2020
UL http://biorxiv.org/content/early/2020/04/30/2020.04.28.065680.abstract
AB Two inhibitory cell types involved in modulating barrel cortex activity and perception during active whisking in adult mice, are the VIP+ and SST+ interneurons. Here we identify a developmental transition point of structural and functional rearrangements onto these interneuron types around the start of active sensation at P14. Using in vivo two-photon Ca2+ imaging, we find that before P14, both interneuron types respond stronger to a multi-whisker stimulus, whereas after P14 their responses diverge, with VIP+ cells losing their multi-whisker preference and SST+ neurons enhancing theirs. Rabies virus tracings followed by tissue clearing, as well as photostimulation-coupled electrophysiology reveal that SST+ cells receive higher cross-barrel inputs compared to VIP+ at both time points. In addition, we also uncover that whereas prior to P14 both cell types receive direct input from the sensory thalamus, after P14 VIP+ cells show reduced inputs and SST+ cells largely shift to motor-related thalamic nuclei.Competing Interest StatementThe authors have declared no competing interest.