RT Journal Article
SR Electronic
T1 Fluorogenic probe for fast 3D whole-cell DNA-PAINT
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.04.29.066886
DO 10.1101/2020.04.29.066886
A1 Chung, Kenny KH
A1 Zhang, Zhao
A1 Kidd, Phylicia
A1 Zhang, Yongdeng
A1 Williams, Nathan D
A1 Rollins, Bennett
A1 Yang, Yang
A1 Lin, Chenxiang
A1 Baddeley, David
A1 Bewersdorf, Joerg
YR 2020
UL http://biorxiv.org/content/early/2020/04/30/2020.04.29.066886.abstract
AB DNA-PAINT is an increasingly popular super-resolution microscopy method that can acquire high-fidelity images at nanometer resolution. It suffers, however, from high background and very slow imaging speed, both of which can be attributed to the presence of unbound fluorophores in solution. We present a fluorogenic DNA-PAINT probe that solves these problems and demonstrate 3D imaging without the need for optical sectioning and a 26-fold increase in imaging speed over regular DNA-PAINT.Competing Interest StatementJ. B. discloses significant financial interest in Bruker Corp. and Hamamatsu Photonics.