@article {Tuttle424432, author = {Adam M. Tuttle and Catherine M. Drerup and Molly H. Marra and Alex V. Nechiporuk}, title = {A specific Ret receptor isoform is required for pioneer axon outgrowth and growth cone dynamics}, elocation-id = {424432}, year = {2018}, doi = {10.1101/424432}, publisher = {Cold Spring Harbor Laboratory}, abstract = {In many cases, axon growth and guidance are driven by pioneer axons, the first axons to grow in a particular region. Despite their dynamic pathfinding capabilities and developmental importance, there are very few pioneer neuron specific markers and thus their in vivo identification and functional interrogation have been difficult. We found that a Ret receptor isoform, Ret51, is highly enriched in peripheral sensory pioneer neurons and is required for pioneer axon outgrowth. Ret null mutant pioneer neurons differentiate normally; however, they displayed defects in growth cone morphology and formation of filopodia before pioneer axon extension prematurely halts. We also demonstrate loss-of-function of a retrograde cargo adaptor, JNK-interacting protein 3 (Jip3), phenocopied many of these axonal defects. We further found that loss of Jip3 led to accumulation of activated Ret receptor in pioneer growth cones, indicating a failure in the clearance of activated Ret from growth cones. Using an axon sever approach as well as in vivo analysis of axonal transport, we showed Jip3 specifically mediates retrograde, but not anterograde, transport of activated Ret51. Finally, live imaging revealed that Jip3 and Ret51 were retrogradely co-transported in pioneer axons, suggesting Jip3 functions as an adapter for retrograde transport of Ret51. Taken together, these results identify Ret51 as a molecular marker of pioneer neurons and elucidate an important isoform-specific role for Ret51 in axon growth and growth cone dynamics during development.}, URL = {https://www.biorxiv.org/content/early/2018/09/21/424432}, eprint = {https://www.biorxiv.org/content/early/2018/09/21/424432.full.pdf}, journal = {bioRxiv} }