PT  - JOURNAL ARTICLE
AU  - Bader Al Alwan
AU  - Karmen AbuZineh
AU  - Shuho Nozue
AU  - Aigerim Rakhmatulina
AU  - Mansour Aldehaiman
AU  - Asma S. Al-Amoodi
AU  - Maged F. Serag
AU  - Fajr A. Aleisa
AU  - Jasmeen S. Merzaban
AU  - Satoshi Habuchi
TI  - Spatial confinement and temporal dynamics of selectin ligands enable stable hematopoietic stem cell rolling
AID  - 10.1101/2020.04.29.069617
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.04.29.069617
4099  - http://biorxiv.org/content/early/2020/05/01/2020.04.29.069617.short
4100  - http://biorxiv.org/content/early/2020/05/01/2020.04.29.069617.full
AB  - Hematopoietic stem/progenitor cell (HSPC) homing is initiated by tethering and rolling of the cells on endothelium through selectin-ligand interactions. Although multiple factors that affect the rolling behaviour of the cells have been identified, molecular mechanisms that enable slow and stable cell rolling remain elusive. Here, using a microfluidics-based single-molecule live cell fluorescence imaging, we reveal that unique spatiotemporal dynamics of selectin ligands on the membrane tethers and slings, which are distinct from that on the cell body, play an essential role in the rolling of the cell. Our results suggest that the spatial confinement of the selectin ligands to the tethers and slings together with the rapid scanning of a large area by the selectin ligands increases the efficiency of selectin-ligand interactions during cell rolling, resulting in slow and stable rolling of the cell on the selectins. Our findings provide novel insights and contribute significantly to the molecular-level understanding of the initial and essential step of the homing.Competing Interest StatementThe authors have declared no competing interest.