PT  - JOURNAL ARTICLE
AU  - Paola Cristina Resende
AU  - Fernando Couto Motta
AU  - Sunando Roy
AU  - Luciana Appolinario
AU  - Allison Fabri
AU  - Joilson Xavier
AU  - Kathryn Harris
AU  - Aline Rocha Matos
AU  - Braulia Caetano
AU  - Maria Orgeswalska
AU  - Milene Miranda
AU  - Cristiana Garcia
AU  - André Abreu
AU  - Rachel Williams
AU  - Judith Breuer
AU  - Marilda M Siqueira
TI  - SARS-CoV-2 genomes recovered by long amplicon tiling multiplex approach using nanopore sequencing and applicable to other sequencing platforms
AID  - 10.1101/2020.04.30.069039
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.04.30.069039
4099  - http://biorxiv.org/content/early/2020/05/01/2020.04.30.069039.short
4100  - http://biorxiv.org/content/early/2020/05/01/2020.04.30.069039.full
AB  - Genomic surveillance has become a useful tool for better understanding virus pathogenicity, origin and spread. Obtaining accurately assembled, complete viral genomes directly from clinical samples is still a challenging. Here, we describe three protocols using a unique primer set designed to recover long reads of SARS-CoV-2 directly from total RNA extracted from clinical samples. This protocol is useful, accessible and adaptable to laboratories with varying resources and access to distinct sequencing methods: Nanopore, Illumina and/or Sanger.Competing Interest StatementThe authors have declared no competing interest.