TY - JOUR T1 - Kinetically distinct phases of tau on microtubules regulate kinesin motors and severing enzymes JF - bioRxiv DO - 10.1101/424374 SP - 424374 AU - Valerie Siahaan AU - Jochen Krattenmacher AU - Amayra Hernandez-Vega AU - Anthony A. Hyman AU - Stefan Diez AU - Zdenek Lansky AU - Marcus Braun Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/09/22/424374.abstract N2 - Tau is an intrinsically disordered protein, which diffuses on microtubules. In neurodegenerative diseases collectively termed tauopathies, tau malfunction and its detachment from axonal microtubules is correlated with microtubule degradation. It is known that tau can protect microtubules from microtubule-degrading enzymes, such as katanin. However, how tau can fulfill such regulative function is still unclear. Using in vitro reconstitution, we here show that tau molecules on microtubules cooperatively form islands of an ordered layer with regulatory qualities distinct from a comparably dense layer of diffusible tau. These islands shield the microtubules from katanin and kinesin-1 but are penetrable by kinesin-8 which causes the islands to disassemble. Our results indicate a new phase of tau, constituting an adjustable protective sheath around microtubules. ER -