TY - JOUR T1 - Physical mechanisms of amyloid nucleation on fluid membranes JF - bioRxiv DO - 10.1101/2019.12.22.886267 SP - 2019.12.22.886267 AU - Johannes Krausser AU - Tuomas P. J. Knowles AU - Anđela Šarić Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/05/02/2019.12.22.886267.abstract N2 - Biological membranes can dramatically accelerate the aggregation of normally soluble protein molecules into amyloid fibrils and alter the fibril morphologies, yet the molecular mechanisms through which this accelerated nucleation takes place are not yet understood. Here, we develop a coarse-grained model to systematically explore the effect that the structural properties of the lipid membrane and the nature of protein-membrane interactions have on the nucleation rates of amyloid fibrils. We identify two physically distinct nucleation pathways and quantify how the membrane fluidity and protein-membrane affinity control the relative importance of those molecular pathways. We find that the membrane’s susceptibility to reshaping and being incorporated into the fibrillar aggregates is a key determinant of its ability to promote protein aggregation. We then characterise the rates and the free energy profile associated to this heterogeneous nucleation process in which the surface itself participates in the aggregate structure. Finally, we compare quantitatively our data to experiments on membrane-catalysed amyloid aggregation of α-synuclein, a protein implicated in Parkinson’s disease that predominately nucleates on membranes. More generally, our results provide a framework for understanding macromolecular aggregation on lipid membranes in a broad biological and biotechnological context.Competing Interest StatementThe authors have declared no competing interest. ER -