TY - JOUR T1 - Functional importance of JMY expression by Sertoli cells in mediating mouse spermatogenesis JF - bioRxiv DO - 10.1101/425082 SP - 425082 AU - Yue Liu AU - Jiaying Fan AU - Yan Yan AU - Xuening Dang AU - Ran Zhao AU - Yimei Xu AU - Zhide Ding Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/09/24/425082.abstract N2 - Sertoli cells are crucial for spermatogenesis in the seminiferous epithelium because their actin cytoskeleton supports vesicle transport, cell junction, protein anchoring and spermiation. Here, we show that junction-mediating and regulatory protein (JMY), an actin regulating protein, also affects endocytic vesicle trafficking and Sertoli cell junction remodeling since disruption of these functions induced male subfertility in Sertoli cell-specific Jmy knockout mice. Specifically, these mice have: a) impaired BTB integrity and spermatid adhesion in the seminiferous tubules; b) high incidence of sperm structural deformity; c) reduced sperm count and poor sperm motility. Moreover, the cytoskeletal integrity in Sertoli cell-specific Jmy knockout mice was compromised along with endocytic vesicular trafficking. These effects impaired junctional protein recycling and reduced Sertoli cell junctions. In addition, JMY interaction with α-actinin1 and Sorbs2 was related to JMY activity and in turn actin cytoskeletal organization. In summary, JMY affects control of spermatogenesis through regulating actin filament organization and endocytic vesicle trafficking in Sertoli cells.ARP3actin-related protein 3BTBblood–testis barrierCKOconditional knock outDHTdihydrotestosteroneEEA-1early endosomal antigenESectoplasmic specializationH&Ehematoxylin and eosinIPimmunoprecipitationMSmass spectrometryJMYjunction-mediating and regulatory proteinSorbs2Sorbin and SH3 domain containing protein 2ZO-1zonula occludens-1 ER -