TY - JOUR T1 - Spatiotemporal contribution of neuromesodermal progenitor-derived neural cells in the elongation of developing mouse spinal cord JF - bioRxiv DO - 10.1101/2020.05.03.075382 SP - 2020.05.03.075382 AU - Mohammed R Shaker AU - Ju-Hyun Lee AU - Kyung Hyun Kim AU - Veronica Jihyun Kim AU - Joo Yeon Kim AU - Ji Yeoun Lee AU - Woong Sun Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/05/04/2020.05.03.075382.abstract N2 - During vertebrate development, the posterior end of the embryo progressively elongates in a head-to-tail direction to form the body plan. Recent lineage tracing experiments revealed that bi-potent progenitors, called neuromesodermal progenitors (NMPs), produce caudal neural and mesodermal tissues during axial elongation. However, their precise location and contribution to spinal cord development remain elusive. Here we used NMP-specific markers (Sox2 and BraT) and a genetic lineage tracing system to localize NMP progeny in vivo. NMPs were initially located at the tail tip, but were later found in the caudal neural tube, which is a unique feature of mouse development. In the neural tube, they produced neural stem cells (NSCs) and contributed to the spinal cord gradually along the AP axis during axial elongation. Interestingly, NMP-derived NSCs preferentially contributed to the ventral side first and later to the dorsal side at the lumbar spinal cord level, which may be associated with atypical junctional neurulation in mice. Our current observations detail the contribution of NMP progeny to spinal cord elongation and provide insights into how different species uniquely execute caudal morphogenesis. ER -