RT Journal Article
SR Electronic
T1 Cell competition corrects noisy Wnt morphogen gradients to achieve robust patterning
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 423814
DO 10.1101/423814
A1 Yuki Akieda
A1 Shohei Ogamino
A1 Hironobu Furuie
A1 Shizuka Ishitani
A1 Ryutaro Akiyoshi
A1 Jumpei Nogami
A1 Takamasa Masuda
A1 Nobuyuki Shimizu
A1 Yasuyuki Ohkawa
A1 Tohru Ishitani
YR 2018
UL http://biorxiv.org/content/early/2018/09/25/423814.abstract
AB Morphogen signaling forms an activity gradient and instructs cell identities in a signaling strength-dependent manner to pattern developing tissues. However, developing tissues also undergo dynamic morphogenesis, which may produce cells with unfit morphogen signaling and consequent noisy morphogen gradient. Here we show that a cell competition-related system corrects such noisy morphogen gradients. Zebrafish imaging analyses of the Wnt/β-catenin signaling-gradient, which acts as a morphogen to establish embryonic anterior-posterior patterning, revealed that unfit cells with abnormal Wnt/β-catenin activity spontaneously appear and produce noise in the Wnt/β-catenin-gradient. Communication between the unfit and neighboring fit cells via cadherin proteins stimulates the apoptosis of the unfit cells by activating Smad signaling and reactive oxygen species production. This unfit cell elimination is required for proper Wnt/β-catenin-gradient formation and consequent anterior-posterior patterning. Because this gradient controls patterning not only in the embryo but also in adult tissues, this system may support tissue robustness and disease prevention.