%0 Journal Article %A Lindsey J. Caldwell %A Nick O. Davies %A Leonardo Cavone %A Karolina S. Mysiak %A Svetlana A. Semenova %A Pertti Panula %A J. Douglas Armstrong %A Catherina G. Becker %A Thomas Becker %T Regeneration of dopaminergic neurons in adult zebrafish depends on immune system activation and differs for distinct populations %D 2018 %R 10.1101/367151 %J bioRxiv %P 367151 %X Adult zebrafish regenerate neurons in their brain, but the extent and variability of this capacity is unclear. Here we ask whether loss of various dopaminergic neuron populations is sufficient to trigger their functional regeneration. Genetic lineage tracing shows that specific diencephalic ependymo-radial glial progenitor cells (ERGs) give rise to new dopaminergic (Th+) neurons. Ablation elicits an immune response, increased proliferation of ERGs and increased addition of new Th+ neurons in populations that constitutively add new neurons, e.g. diencephalic population 5/6. Inhibiting the immune response attenuates neurogenesis to control levels. Boosting the immune response enhances ERG proliferation, but not addition of Th+ neurons. In contrast, in populations in which constitutive neurogenesis is undetectable, e.g. the posterior tuberculum and locus coeruleus, cell replacement and tissue integration are incomplete and transient. This is associated with loss of spinal Th+ axons, as well as permanent deficits in shoaling and reproductive behaviour. Hence, dopaminergic neuron populations in the adult zebrafish brain show vast differences in regenerative capacity that correlate with constitutive addition of neurons and depend on immune system activation. %U https://www.biorxiv.org/content/biorxiv/early/2018/09/25/367151.full.pdf